Mark Hirst allegedly posted a video online during which he is said to have made threatening remarks about the complainers at the ex-SNP leader’s trial at the High Court in Edinburgh in March.
That offence is said to have been committed at his home near Jedburgh on March 29.
The 50-year-old – formerly employed by nationalist MSPs at Holyrood including Midlothian South, Tweeddale and Lauderdale representative Christine Grahame – pleaded not guilty through lawyer Michael Gallen to that charge.
A trial date was set for January 7 next year at Jedburgh Sheriff Court.
It is alleged that he behaved in a threatening or abusive manner by creating and uploading to the internet a video recording in which he uttered threatening remarks directed at witnesses for the prosecution at Mr Salmond’s trial.
The West Lothian-born 65-year-old, Scottish Government first minister from 2007 to 2014, was cleared of 13 charges of sexual assault against nine women following his trial in March.